Offer & Services

Development of molecular assays & high throughput screening (typically down to 384-well plate format)

  • stages: assay development, miniaturisation, automation, primary screening, hits validation, orthogonal assays, data analysis
  • biological targets and models: RNA, proteins, plant cells, animal (including human) 2D and 3D cellular models, patient-derived samples
  • modes of detection: absorbance, fluorescence, bioluminescence and derivatives as well as advanced multiparametric imaging
  • libraries of chemical compounds: EU-OPENSCREEN diversity library of 100k commercially available compounds (including 5k pilot library for testing), EU-OPENSCREEN “pilot” library of 5000commercial “bioactives” libraries (PRESTWICK and LOPAC), EU-OPENSCREEN diversity library of 100k compounds, academic compound libraries of EU-OPNESCREEN and POL-OPENSCREEEN as well as any libraries provided by the user)
  • Examples of more unique assays:
    • a) assays and screening for RNA binders (esp. binders of specific 3D RNA structures),
    • b) multiparametric assays for pairs of enzymes in one assay,
    • c) bioprofiling (evaluation of ROS generation and luciferases inhibition by compounds – for higher reliability and off-target elimination)
    • d) advanced multiparametric imaging for more reliable, early-stage safety evaluation nand mechanism deconvolution (e.g. cell painting – hyperlink to the article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/)
Fig. 2 a)example workflow
Fig. 2 a)example workflow
Fig 2. b) photograph of stained HEK cells (4 channels)
Fig 2. b) photograph of stained HEK cells (4 channels)
Fig. 2. c) photograph of stained A549 cells (4 channels)
Fig. 2. c) photograph of stained A549 cells (4 channels)

Fig. 2 Cell painting experiment a) example workflow b) photograph of stained HEK cells (4 channels) c) photograph of stained A549 cells (4 channels)
Medicinal chemistry:

  • hit analysis, selection and design of targeted libraries for structure-activity-relationship evaluation
  • Molecule (hit) optimization & tool compounds development
  • Synthesis (organic synthesis, biosynthesis, biocatalysis, semi-synthesis) of complex, difficult to prepare analogues (e.g. analogues of natural comuponds)
  • Isolation and purification of chemicals

Advanced image and data analysis
Technology co-development and testing (particularly in collaboration with industry)
scientific expertise & consulting in preparation of concepts of research projects & research grant applications involving experiments on our infrastructure to suport acquisition of research funding.
Other services (associated):

  • Ultra-resolution fluorescence microscopy (STED & MINFLUX down to < 5 nm, 2D and 3D, multicolour, FLIM, FCS)
  • Design & development of fluorescent & bioluminescent probes & covalent inhibitors
  • Protein production & crystallisation
  • Mass spectrommetry & NMR

Image-based flow cytommetry & fluorescence-assisted cell sorting (FACS)